Table 2. RNA dissociation rate constants.
| Protein | Nucleotide | k 1 (× 10−3 s−1) | Amplitude | k 2 (× 10−3 s−1) | Amplitude |
|---|---|---|---|---|---|
| S411A | None | 45 ± 0.2 | 52% | 4 ± 0.1 | 48% |
| ATPγS | 68 ± 0.9 | 42% | 2 ± 0.04 | 58% | |
| ADP | 70 ± 0.9 | 62% | 6 ± 0.1 | 38% | |
| S411L | None | 27 ± 0.2 | 52% | 2 ± 0.04 | 48% |
| ATPγS | 210 ± 1 | 81% | 10 ± 0.1 | 19% |
Rate constants for the dissociation of S411A and S411L measured using stopped-flow fluorescence spectroscopy as described in Materials and Methods. Dissociation was measured from a 5΄ppp10L hairpin RNA in all cases. The data were fit to a biphasic exponential decay equation. Values represent mean ± SD (n = 3).