Figure 4.

HBV surface antigen (HBsAg) knockdown kinetics and RNAi trigger guide strand decay in mouse liver. Isotonic glucose, ARC-520 or ARC-EX1 alone was administered as a single dose at 8 mg/kg to mice. (A) Serum levels of HBsAg were evaluated by ELISA. (B) Mice were sacrificed at multiple time points and livers evaluated by PNA assay for non-phosphorylated full-length guide strands (non-P FL GS) or 5΄-phosphorylated full-length guide strands (5΄P FL GS). Quantitation of guide strands was based on a standard curve, and indicated as ng of guide strands per gram of liver tissue. LLOQ, lower limit of quantitation (0.1 ng/g).