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Dose-dependent correlation between HBsAg levels and 5΄-phosphorylated RNAi trigger guide strands. Mice were administered isotonic glucose or a single dose of 2–8 mg/kg ARC-520. On day 8, HBsAg levels were evaluated in serum and AD0009 + AD0010 5΄-phosphorylated FL guide strands were assessed in liver. Quantitation of guide strands was based on a standard curve, and indicated as ng of guide strands per gram of liver tissue.
