Figure 3.

SELEX oligonucleotide pool binding by SELEX round. (A) Flow cytometry binding assays carried out by fluorescently labeling the SELEX pools from each round (SELEX0 is the initial randomized pool) and measuring the amount of fluorescent oligonucleotide bound by the indicated yeast surface displayed enzyme via flow cytometry. Each enzyme's affinity for the targets in the indicated SELEX pool increased with each round with the exception of I-CpaMVP. (B) A summary plot of the binding data, plotting the median fluorescence intensity of bound oligonucleotide by SELEX round. Significant increases in binding occurred over the five rounds of SELEX.