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. 2016 Oct 7;45(3):e14. doi: 10.1093/nar/gkw900

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Effect of including negative references in AmoA ROCker profiles for simulated shotgun data sets of different read lengths. Simulated shotgun data sets of 80, 100, 150, 200 and 250 bp read length were searched against (target) AmoA reference sequences using BLASTx. Panel A shows the results of using ROCker archaeal AmoA profiles, including bacterial AmoA and PmoA as negative references, and e-values for filtering the simulated data sets. Panel B shows the results of using ROCker bacterial AmoA profiles, including archaeal AmoA and PmoA as negative references, and e-values.