FIG. 2.
Complementation of the bisC mutation. (A) The sequence upstream of the bisC gene is shown. Two putative Shine-Dalgarno sequences are indicated as empty and shaded boxes, respectively. Pu-tative TTG and ATG initiation codons are in bold. bisC(ORF1) starts at the ATG codon, and bisC(ORF2) starts at the TTG codon. (B) Growth experiments were carried out as described for panel A except that glycerol was used as a carbon source and arabinose was present to induce expression of plasmid-borne genes. The strains analyzed were JB08 (metB1 msrA msrB bisC/pBAD24), JB308 (metB1 msrA msrB bisC/pBis2), and JB309 (metB1 msrA msrB bisC/pBis1).