TABLE 1.
Strains and plasmids
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| DH5α | φ80d lacZΔM15 recA1 endA1 gyr A96 thi-1 hsdR17 supE44 relA1 deoR Δ(lacZYA-argF)U169 | Promega |
| BL21 (DE3) | F−ompT hsdS (rB− mB−) gal dcm(DE3) | Novagen |
| BW25113 | lacIqrrnBT14 ΔlacZWJ16hsdR514 ΔaraBADAH33 ΔrhaBADLD78 | 2 |
| MC4100 | araD139 Δ (argF-lac)U169 rpsL150 relA1 thi fibB5301 deoC1 ptsF25 rbsR | Promega |
| HMS174 (DE3) | F−recA hsdR (rK12− mK12+) Rifr(DE3) | Novagen |
| LCB303 | MC4100 metB1 | Lab collection |
| BE54 | MC4100 metB1 msrA::SpcrmsrB::Cmr | 7 |
| Tp1000 | MC4100 ΔmobAB Kanr | 18 |
| BE72 | MC4100 metB1 msrA::SpcrmsrB::Cmr ΔmobAB::Kanr | This study |
| JB02 | MC4100 metB1 msrA::Spcr | This study |
| JB07 | BW25113 bisC::Kanr | This study |
| JB08 | MC4100 metB1 msrA::SpcrmsrB::CmrbisC::Kanr | This study |
| JB09 | MC4100 metB1 bisC::Kanr | This study |
| JB11 | MC4100 metB1 trxA::Kanr | This study |
| JB308 | MC4100 metB1 msrA::SpcrmsrB::CmrbisC::Kanr/pBis2 | This study |
| JB309 | MC4100 metB1 msrA::SpcrmsrB::CmrbisC::Kanr/pBis1 | This study |
| Plasmids | ||
| pJBis | pET21a+, BisCorf2 (His6 tag), Ampr | This study |
| pBis1 | pBad24, BisC(orf1), Ampr | This study |
| pBis2 | pBad24, BisC(orf2), Ampr | This study |
| pMsrA | pUC18, MsrA Ampr | 7 |
| pKD46 | Ampr | 2 |
| pKD4 | Kanr | 2 |