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. 2017 Apr 11;12(4):e0175459. doi: 10.1371/journal.pone.0175459

Fig 3. Effects of miR-150 on human keratinocytes and expression of HIF-1α and VEGFA in hypoxic condition.

Fig 3

The cell viability of HaCaT cells (A) and HKCs (C) transfected with NC mimics/miR-150 mimics in 1% O2 condition or 20% O2 condition was monitored using CCK-8 assays. The cell proliferation of HaCaT cells (B) and HKCs (D) transfected with NC mimics/miR-150 mimics in 1% O2 condition or 20% O2 condition was monitored using BrdU-ELISA assays. (E) and (F) The protein expression of HIF-1α and VEGFA in HaCaT cells and HKCs transfected with NC mimics/miR-150 mimics in 1% O2 condition or 20% O2 condition was monitored using Western blot assays. (G) and (H) The protein expression of HIF-1α and VEGFA in HaCaT cells and HKCs transfected with NC mimics/miR-150 mimics or NC inhibitor/miR-150 inhibitor was monitored using Western blot assays. The data are presented as mean ± SD of three independent experiments. *P<0.05, **P<0.01.