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. 2017 Apr 11;12(4):e0175459. doi: 10.1371/journal.pone.0175459

Fig 6. The role of miR-150/HIF-1α and miR-150/VEGFA in regulating HKCs proliferation.

Fig 6

(A, B) pcDNA3.1/HIF-1α and pcDNA3.1/VEGFA was transfected into HKCs to achieve ectopic expression of HIF-1α or VEGFA, respectively. The protein expression of HIF-1α and VEGFA was verified using Western blot assays. (C) HaCaT cells were co-transfected with pcDNA3.1/HIF-1α and NC mimics/miR-150 mimics, and the cell viability was monitored in each group using CCK-8 assays. (D) HKCs were co-transfected with pcDNA3.1/VEGFA and NC mimics/miR-150 mimics, and the cell viability was monitored in each group using CCK-8 assays. (E) HKCs were co-transfected with pcDNA3.1/HIF-1α and NC mimics/miR-150 mimics, and the cell proliferation was monitored in each group using BrdU-ELISA assays. (D) HKCs were co-transfected with pcDNA3.1/VEGFA and NC mimics/miR-150 mimics, and the cell proliferation was monitored in each group using BrdU-ELISA assays. The data are presented as mean ± SD of three independent experiments. *P<0.05, **P<0.01.