Table 2. Comparison of immunological variables between patients with and without acute GVHD.
| Immunological variables ^ | Pre-SCT | aGVHD onset* | ||||
|---|---|---|---|---|---|---|
| a GVHD | a GVHD | |||||
| Yes | No | p | Yes | No | p | |
| CD4+(total) | 268 (57–1215) | 228 (4–1118) | 0,59 | 100 (1–1174) | 24 (0–405) | 0,07 |
| RTE | 10 (3–123) | 25 (0–94) | 0,64 | 4 (0–115) | 0 (0–24) | 0,01 |
| CD4+naïve | 37 (4–316) | 56 (1–205) | 0,84 | 8 (0–165) | 0 (0–34) | 0,005 |
| CD4+TCM | 113 (37–1092) | 98 (2–233) | 0,30 | 25 (0–254) | 8 (0–86) | 0,03 |
| CD4+TEM | 63 (2–210) | 59 (2–769) | 0,90 | 45 (0–986) | 16 (0–318) | 0,26 |
| CD4+TEMRA | 2 (0–14) | 2 (0–209) | 0,66 | 2 (0–36) | 0 (0–20) | 0,02 |
| Treg (total) | 16 (3–429 | 17 (1–45) | 0,97 | 7 (0–30) | 2 (0–11) | 0,005 |
| Treg naïve | 1 (0–19) | 3 (0–12) | 0,41 | 2 (0–6) | 0 (0–5) | 0,02 |
| TregCM | 8 (2–18) | 5 (0–21) | 0,29 | 3 (0–17) | 0 (0–4) | 0,006 |
| TregEM | 6 (0–25) | 6 (1–22) | 0,90 | 3 (0–17) | 1 (0–7) | 0,01 |
| CD8+(total) | 151 (10–870) | 147 (1–998) | 0,84 | 44 (1–1109) | 30 (0–1138) | 0,27 |
| CD8+ naïve | 17 (1–126) | 16 (0–105) | 0,80 | 4 (0–85) | 2 (0–21) | 0,25 |
| CD8+TCM | 11 (1–60) | 9 (0–83) | 0,95 | 3 (0–34) | 1 (0–17) | 0,08 |
| CD8+TEM | 63 (1–520) | 29 (0–566) | 0,30 | 26 (0–360) | 21 (0–669) | 0,50 |
| CD8+TEMRA | 44 (1–446) | 65 (0–469) | 0,82 | 8 (0–603) | 5 (0–434) | 0,37 |
| CD19+(total) | 22 (0–102) | 32 (0–145) | 0,50 | 1 (0–11) | 0 (0–19) | 0,27 |
| Immature CD19+ | 4 (0–38) | 9 (0–95) | 0,19 | 0 (0–7) | 0 (0–6) | 0,81 |
| Mature CD19+ | 10 (0–93) | 16 (0–100) | 0,88 | 1 (0–11) | 0 (0–15) | 0,40 |
| CD19+ naïve | 5 (0–50) | 4 (0–93) | 0,98 | 0 (0–9) | 0 (0–11) | 0,70 |
| UM CD19+ | 0 (0–12) | 0 (0–4) | 0,41 | 0 (0–1) | 0 | 0,40 |
| SM CD19+ | 1 (0–24) | 1 (0–7) | 0,67 | 0 (0–2) | 0 (0–1) | 0,80 |
| M/DN CD19+ | 0 (0–7) | 1 (0–5) | 0,50 | 0 (0–1) | 0 (0–1) | 0,85 |
| TRECs/ml | 63 (0–1360) | 57 (0–1960) | 0,78 | 82 (0–1769) | 23 (0–885) | 0,34 |
| KRECs/ml | 5099 (0–23957) | 9319 (0–79533) | 0,04 | 191 (0–3914) | 78 (0–9764) | 0,59 |
SCT = stem cell transplantation; aGVHD = acute GVHD; RTE = recent thymic emigrants; CM = central memory; EM = effector memory; TEMRA = terminally differentiated effector memory; UM = unswitched memory; SM = switched memory; M/DN = memory double negative; TRECs = T-cell receptor excision circles; KRECs = k-deleting recombination excision circles
^ Lymphocyte counts are expressed as cells/μl (median; range). Significant differences in percentage of lymphocyte subsets are reported in the section “Results”.
*Values at day+28 were analysed for patients without aGVHD.