Table 1. Antibodies used for detecting viral and protozoal antigens.
Infectious agent | Clone | Specificity | Supplier/Reference | Clonality and source | Dilution |
---|---|---|---|---|---|
Canine distemper virus | anti-canine distemper nucleoprotein antibody (#25) | Nucleoprotein | Prof. Örvell a/Baumgärtner [49] | Polyclonal rabbit | 1:2000 |
Influenza A | HB65 | Nucleoprotein | European Veterinary Laboratory b/van Baalen [50] | Monoclonal mouse | 1:200 |
Parvovirus | CPV1-2A1 | Unknown | Custom Monoclonal Antibodies International c/Kipar [51] | Monoclonal mouse | 1:200 |
Feline leukaemia virus | C11D8 | Glycoprotein 70 | Custom Monoclonal Antibodies International d/Elder [52] | Monoclonal mouse | 1:200 |
Borna disease virus | Bo18 | Nucleoprotein | Dr. Herzog d | Monoclonal mouse | 1:500 |
Tick-borne encephalitis | K-D-3.BA | Not stated | Prof. Holzmannf/Weissenböck [53] | Polyclonal rabbit | 1:300 |
Toxoplasma gondii | Not stated | Quartett g/Klein [54], Brack [55] | Polyclonal rabbit | 1:75 | |
Neospora caninum | Not stated | Dr. Schares h | Monoclonal mouse | 1:2 | |
Canine adenovirus 1 | The whole CAV1 tribe “Mirandola” | Chemicon i | Monoclonal mouse | 1:250 | |
Listeria monocytogenes 1,4 | The whole organism | Difco Laboratories j | Polyclonal rabbit | 1:2000 |
CPV, canine parvo virus; Bo, Borna disease;
a Central Microbiological Laboratory of Stockholm county, Stockholm Council, Sweden;
b Woerden, the Netherlands;
C Sacramento, California, USA
dInstitute of Virology, Justus Liebig University, Giessen, Germany;
e Vienna, Austria;
f Berlin, Germany;
g Munich, Germany;
h Institute of Epidemiology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Greifswald—Insel Riems, Germany;
i Hamburg, Germany;
j Detroit, USA