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. 2017 Apr 11;12(4):e0175338. doi: 10.1371/journal.pone.0175338

Table 1. Loci targeted for PCR using sourced primers.

Forward (-F) and reverse (-R) primer directions indicated by suffix. Original primer sequences modified by addition of 17 bp vector M-13 5´ tails (tail sequences not shown here, refer to Materials and methods).

Locus Primer name Primer sequence 5'-3' Source
atpF intron TeaCpSSR27FP AATGCCGAATCGACGACCTA [17]
TeaCpSSR27RP CAATGGTCCCTCTACGCAAT
matK 390-F CGATCTATTCATTCAATATTTC [20]
1326-R TCTAGCACACGAAAGTCGAAGT
3F-Kimf CGTACAGTACTTTTGTGTTTACGAG
1R-Kimr ACCCAGTCCATCTGGAAATCTTGGTTC
ndhK-ndhC TeaCpSSR29FP GGTACCAATCCATAACGATC [17]
TeaCpSSR29RP GCGCTAGTTTTTGTTGTTTT
psbE-petL TeaCpSSR31FP GGTCGTGGAATGCTTTTCTT
TeaCpSSR31RP TCCACGAATCTCAATGACCA
ETS Rets4-F TTGGCTACGCGAGCGCATGAG [21]
18S-R AGACAAGCATATGACTACTGGCAGG [22]
ITS 26SE TAGAATTCCCCGGTTCGCTCGCCGTTAC [23]
S3 AACCTGCGGAAGGATCATTG [24]
ITS 5a–F TATCATTTAGAGGAAGGAG [25]
ITS 4—R GCATATCAATAAGCGGAGGA