Figure 4. Treatment with cell-permeable octyl-D2-HG increased heterochromatin silencing.
(A) Images of wild type (JRY10790) CRASH assay reporter colonies that were grown on CSM-Trp 3% Glycerol (untreated) or with 100 µM octyl-D2-HG added to the medium (Left Panel). Plots of the frequency of silencing loss calculated using MORPHE (Right Panel). (B) Images of dld2Δ (JRY10733) CRASH assay reporter colonies that were grown on CSM-Trp 3% Glycerol (untreated) or with 100 µM octyl-D2-HG added to the medium (Left Panel). Plots of the frequency of silencing loss from colonies calculated using MORPHE. The data in Figure 4A and B were from independent experiments conducted on different days with different batches of media. MORPHE-based comparisons should be restricted to experiments performed in parallel at the same time (Liu et al., 2016).