Table 1.
Primers used for cloning DNA fragments, screening ES cells, or genotyping by PCR.
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Target region
Exon10 Fw 5’–ATACGAAGTTATCACCGAACCTAGCCCATATTT–3’ Exon10 Rv2 5’–ACGAAGTTATGTCGACGACTGAGCATTGCTGTT–3’ |
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Long arm region
Long arm Fw1 5’–CGAATCAAGCTGTTTGGTCCATTCTCTGCTCCA–3’ Long arm Rv 5’–ACGAAGTTATGGTACGGTCAACTTGAAGAAGTA–3’ |
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Short arm region
Short arm Fw 5’–TAGGAACTTCCTCGAAATTCAGTGCTTAGAAGT–3’ Short arm Rv1 5’–GCGCGCCTTTCTCGAACACTGTGTACAGTGACA–3’ |
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Long-arm probe
larm16380Fw 5’–CTGCCTCAGCTTCCTGAGTG–3’ larm17196Rv 5’–CATGTCAGATCAGACAGTTC–3’ |
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Short-arm probe
sarm26294Fw 5’–CTGAGCTATGTACTGGATGC–3’ AscI-sarmRv2 5’–TGAAGAGGCGCGCCCAGAGACAGAAAAAGCAC–3’ |
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ES cells first screening
PGK S1 5’–CCTCCCCTACCCGGTAGAATTGACC–3’ GL1 typing RV2 5’–GAACTGTCAGATTTGGTGACACAGAAAGGC–3’ |
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ES cells second screening
3rdlox Fw 5’–CCACCCGACCCCTGCCAGAACATAATGCTCTCTTGCATC–3’ 3rdlox Rv 5’–GCTGTCGCCAGAGGAGAGAGTGGGTGCTTACTTAC–3’ |
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Eogt mice genotyping
3rdloxFw 5’–CCACCCGACCCCTGCCAGAACATAATGCTCTCTTGCATC–3’ 3rdloxRv2 5’–GCTGTCGCCAGAGGAGAGAGTGGGTGCTTACTTAC–3’ 25307Rv 5’–CCAAGGCGGTCTTGGCCCAT–3’ |
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RT-PCR for Eogt
Exon 9 Fw 5’– AGGCTACACGCAGCTCAATT –3’ Exon 11 Rv 5’– AGAAGCCGTGTTTTCGTTGC –3’ |
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qRT-PCR for Eogt
Exon 1 Fw 5’–AAGCTGCAGGTCCGTGAAAA–3’ Exon 2 Rv 5’–TAGGTTAGGCTACCGCGTCT–3’ |
Underlined sequences are 15 bp homologous overlaps required for In-Fusion cloning.