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. 2017 Mar 14;6:e23001. doi: 10.7554/eLife.23001

Figure 1. Biosynthesis of forskolin in the root cork cells of C. forskohlii.

Figure 1.

(A) Scheme showing the structures of the diterpene precursor 13R-manoyl oxide, deacetylforskolin and forskolin on a background of root cork cells with forskolin containing oil bodies. (B) Transcript profiles of biosynthetic candidate genes in selected tissues of C. forskohlii as shown on the illustrations.

DOI: http://dx.doi.org/10.7554/eLife.23001.003

Figure 1—source data 1. cDNAs identified in the C. forskohlii root cork transcriptome and cloned during this work, with the GeneBank accession numbers.
DOI: http://dx.doi.org/10.7554/eLife.23001.004
elife-23001-fig1-data1.docx (13.2KB, docx)
DOI: 10.7554/eLife.23001.004
Figure 1—source data 2. Table of FPKM (Fragments Per Kilobase of transcript per Million mapped reads) values of the first 20 most abundant cDNAs identified in the root cork transcriptome library.
cDNAs involved in terpenoid metabolism are marked in bold.
DOI: http://dx.doi.org/10.7554/eLife.23001.005
elife-23001-fig1-data2.docx (20.3KB, docx)
DOI: 10.7554/eLife.23001.005
Figure 1—source data 3. Table of primers used in this study.
Construction of plasmids for expression of CfTPS2, CfTPS3, CfTPS1 is described in Andersen-Ranberg et al. (2016). U (uracil, marked in bold), represents the cleavage site, used in the USER cloning (Nour-Eldin et al., 2006).
DOI: http://dx.doi.org/10.7554/eLife.23001.006
elife-23001-fig1-data3.docx (30.4KB, docx)
DOI: 10.7554/eLife.23001.006