Figure 12. Phylogenetic tree of CfACT encoding candidate genes together with BAHD family acyltransferase representatives from all clades according to D'Auria (2006).
Accession numbers of the non-Coleus forskohlii selected protein sequences are shown next to the tree taxon names, while C. forskohlii peptide accession numbers are provided in Figure 1—source data 1. The analysis only includes functionally characterized members. Coleus forskohlii enzymes are indicated by a solid black triangle. The majority of the selected CfACTs belong to Clade III, which includes mainly members which accept a diverse range of hydroxylated substrates and use acetyl-CoA as the main acyl donor (D'Auria, 2006). Interestingly, the ACTs known to be involved in Taxol biosynthesis belong to Clade V.