Figure 9. Conserved expression of Emx2 in the three chicken maculae.
(A–C’) Anti-Emx2 immunostaining of the chicken utricle (A,A’), saccule (B,B’) and lagena (C,C’) at E12. (A–C) Anti-spectrin staining (red) shows opposite hair bundle orientation across the LPR (yellow line). In the same regions shown in (A), (B), and (C) but at the level of the cell body, the border (green line) of the Emx2-positive region (green) is restricted to only one side of the LPR: lateral region of the utricle (A’), inner region of the saccule (B’), and proximal region of the lagena, which is closer to the cochlea/basilar papilla (BP) (C’), respectively (n = 3). (D) Schematic diagrams of the chicken inner ear and its three macular organs.