Figure 10.

Curcumin inhibits formation of eicosanoids in activated human leukocytes. Leukocytes were isolated and processed in the presence of curcumin (40 μM). LC-SRM-MS chromatograms for the analysis of AMPP derivatized eicosanoids are shown for leukocytes isolated in the absence (A, B) or presence (C) of curcumin (40 μM) and activated with LPS (10 μg/ml) for 15 min (A) or 5 h (B, C). All samples were treated with A23187 (5 μM) for 15 min before the end of incubation. The peak eluting after LTB₄ is 5,12-diHETE, the peak eluting before PGE₂ is 20-hydroxy-LTB₄. The ion chromatograms are shown in relative intensity to the highest peak in A. The absolute intensities for each ion chromatogram are given, and the insets show the elution of HKE₂ and HKD₂ in an expanded view.