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LC-SRM-MS analysis of underivatized and AMPP-derivatized HK eicosanoids formed in the reaction of 5S-HETE with COX-2 in vitro. A, B) HKE2 was analyzed directly in negative ion mode (A) or after derivatization with AMPP in positive ion mode (B). C, D) The same analyses were performed with HKD2 (C) and AMPP-HKD2 (D). E, F) LC-ESI-MS2 spectra of AMPP-derivatized HKE2 (E) and HKD2 (F).
