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Effect of SC560 on eicosanoid formation in human leukocytes. HKE2 (A) and HKD2 (B), as well as 5-HETE (C), LTB4 (D), and PGE2 (E), were quantified using LC-SRM-MS analyses after derivatization with AMPP. SC560 (1 μM) was added 15 min before the stimulation with LPS (10 μg/ml) and A23187 (5 μM) for additional 15 min. Veh, vehicle. *P < 0.05 in comparison to stimulated and vehicle-treated sample.
