Figure 3.

Reduced mitochondrial activity in colonic ρ⁰ cells promotes inflammatory pathways with IL-8 as top expressed transcript. A, B) Venn diagrams represent number of transcripts commonly or uniquely expressed in control (con) and colonic ρ⁰ cells without (A) or with (B) TNF-α (6 h) treatment (FDR < 0.05, EBseq). C) Top canonical pathways activated in colonic ρ⁰ cells and TNF-α-treated (con and ρ⁰) cells relative to con cells (FDR < 0.05, IPA). D) Top canonical pathways altered in ρ⁰ cells and human IBD colon (GSE4183) (FDR < 0.05, IPA). E) Top transcripts upregulated in inflammatory response signaling in colonic ρ⁰ cells and TNF-α-treated (con and ρ⁰) cells relative to control cells (FDR < 0.05, IPA). F) Top transcripts upregulated in inflammatory response signaling in ρ⁰ cells and human IBD colon (GSE4183) (FDR < 0.05, IPA). G) Total RNA was extracted from HCT116 con, ρ⁰ cells, or cells incubated with AntiA and basal and TNF-induced (2 h) IL-8 mRNA levels were quantified using qPCR (n = 3). *P < 0.05, compared to con; ^#P < 0.05 compared to TNF-α alone, ANOVA.