Figure 3.

HG induces chromatin remodeling in gene promoters of profibrotic factors. A–C) ChIP assays showing that HG induces pCREB(S133) or pp65(S276) binding, CBP recruitment, H3K9/14 hyperacetylation, and RNA Pol II occupation in the TSS region in Tgfb1 (A), Ctgf (B), and Tgfb3 (C) promoters in MCs, as well as in purified glomeruli from streptozotocin (STZ)-induced diabetic DBA/2J and ob/ob BTBR mice, as indicated. D, E) Illustration of the chromatin remodeling events around NF-κB motif and CREB motif in Tgfb1and Ctgf promoters (D) or Tgfb3 (E) promoter. *P < 0.05; **P < 0.01; ***P < 0.001 vs. corresponding controls (LG, Ctrl, or ob/+); n ≥ 3 in each assay.