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. 2017 Feb 1;31(5):2065–2075. doi: 10.1096/fj.201601116R

Figure 6.

Figure 6.

Essential roles of the cAMP-PKA pathway in HG-induced histone acetylation and chromatin remodeling in fibrogenic gene promoters. MCs were exposed to LG and HG conditions in the presence or absence of H89, KH7, or 2′5′-dideoxyadenosine (ddAdo) inhibitor. A, B) Western blots analyzing phospho-p65(S276) and phospho-p65(S536) (A) or phospho-CREB(S133) and total CREB (B) in cell lysates. C, D) Coimmiunoprecipitation assays measuring the recruitment of CBP by phospho-p65(S276) (C) or phospho-CREB(S133) (D) in these lysates. E) ChIP assays measuring HG-induced H3K9/14 acetylation, CBP recruitment, and RNA Pol II occupation in Tgfb1 promoter. F) ChIP assays assessing HG-induced H3K9/14 acetylation in Tgfb3 and Ctgf promoters. G, H) Western blots analyzing TGF-β1 (G) and fibronectin (H) proteins in these cell lysates. IB, immunoblot. FN, fibronectin. *P < 0.05; **P < 0.01; ***P < 0.001 vs. corresponding LG; n = 3.