Figure 2. Effects of iron and folate supplementation on embryo size and developmental stage.

A. Comparison of crown to rump length (A) and somite numbers (B) of embryos from Fpn1^ffe/ffe mutant embryos (White Bars in A) and wildtype littermates (Colored Bars in A) dissected at 9.5 dpc from dams supplemented with control (yellow), high folic acid (10 ppm, orange), high iron (0.5% carbonyl iron, blue) or high folic acid and iron (purple) diets for 4 weeks before mating. C. Comparison of weights of Fpn1^ffe/ffe mutant embryos and wildtype littermates dissected at 11.5 dpc from dams fed the various diets for 4 weeks before mating. Statistical significance was determined by unpaired t-test in A and C, and by 2- factor ANOVA with post-hoc Sidak's multiple comparisons test in B. P-values: ≤0.05 *, ≤0.01 ** or non significant (ns).