Figure 5. Forebrain truncations in Fpn1^ffe/ffe mutant embryos are rescued by supplementation with a high iron diet.

A. In situ hybridization to detect Six3 expression in 9.5 dpc embryos. The forebrain was measured from the rostral point of the optic vesicle (stained by Six3) to the most rostral point of the forebrain as indicated by white line. B. Forebrain measurements in 9.5 dpc wildtype (Fpn1^+/+) and Fpn1^ffe/ffe embryos from dams fed control (yellow bar), high folic acid (10 ppm, orange bar), high iron (0.5% carbonyl iron, blue bar) or high folic acid and iron (purple bar) diets for 4 weeks before mating. Statistical significance was determined by the unpaired t-test. P-values: ≤0.0005 *** or non significant (ns). The number of samples represented in each group is indicated.