. Author manuscript; available in PMC: 2018 Jan 30.

Published in final edited form as: Birth Defects Res. 2017 Jan 30;109(2):81–91. doi: 10.1002/bdra.23542

Table 1. Mild macrocytic anemia occurs with high iron diet.

Hematological findings from CBC analyses of whole blood obtained from wildtype (WT) or Fpn1^ffe/+ females fed control, high folic acid (10 ppm), high iron (0.5% carbonyl iron) or high folic acid and iron diets for 6 weeks. Each group represents values from 3 samples and significance was determined by 2-factor ANOVA.

	Control		High Folic Acid		High Iron		High Folic Acid and High Iron		2- Factor Anova P value
Characteristic	Wildtype	Fpn1^ffe/+	Wildtype	Fpn1^ffe/+	Wildtype	Fpn1^ffe/+	Wildtype	Fpn1^ffe/+	Genotype	Diet	Interaction
Red blood cell count, ×10⁶ cells/μL	9.43 ± 0.31	9.54 ± 0.10	9.55 ± 0.24	9.93 ± 0.41	8.90 ± 0.13	9.01 ± 0.08	9.21 ± 0.07	8.97 ± 0.07	0.58	0.01	0.57
Hemoglobin, g/dL	15.23 ± 0.37	15.25 ± 0.03	15.33 ± 0.45	15.33 ± 0.40	15.20 ±0.50	15.45 ± 0.09	15.73 ± 0.27	15.35 ± 0.03	0.69	0.65	0.55
Hematocrit, %	51.37 ± 1.38	51.15 ± 0.03	52.13 ± 1.24	53.83 ± 1.34	50.90 ± 0.92	52.15 ±0.61	53.33 ± 0.38	51.80 ± 0.12	0.65	0.22	0.31
Mean corpuscular volume, fL	54.47 ± 0.38	53.65 ± 0.61	54.57 ± 0.33	54.27 ± 0.98	56.27 ± 1.10	57.90 ± 0.17	57.93 ± 0.15	57.75 ± 0.61	0.85	<0.0001	0.27
Mean corpuscular hemoglobin, pg	16.20 ± 0.15	16.00 ± 0.17	16.03 ± 0.18	15.97 ± 0.32	17.17 ± 0.19	17.20 ± 0.06	17.07 ± 0.24	17.15 ± 0.09	0.78	<0.0001	0.88
Red cell distribution width, %	12.73 ± 0.18	13.65 ± 0.55	12.87 ± 0.15	13.17 ± 0.09	13.23 ± 0.55	12.20 ± 0.06	13.03 ± 0.13	15.30 ± 0.58	0.03	0.005	0.003