FIGURE 5.

Folate and ferritin levels in dams and embryos with high iron and/or folic acid supplementation. A: Determination of folate levels in whole blood from pregnant wild type (WT) or Lrp2^null/+ dams at 9.5 or 11.5 dpc. Dams were fed control (yellow), folic acid (FA; orange), high iron (blue) or high iron and folic acid (purple) diets for 4 weeks before mating or the high iron diet along with folic acid supplemented by daily injection of 25 mg/kg folic acid between 5.5 to 9.5 dpc (high iron + IP FA; blue/orange stripes). B: Determination of folate levels in 11.5 dpc Lrp2^null/null mutant embryos and wild type (Lrp2^+/+) littermates from Lrp2^null/+ dams subjected to the various treatments. C: Ferritin levels in serum from pregnant dams fed the indicated diets was determined by ELISA and served as a proxy for stored iron levels. Statistical significance was determined by two-factor ANOVA with post-hoc Tukey’s multiple comparisons test. p-values: ≤0.01 (**), ≤0.001 (***), ≤0.0001 (****) or non significant (ns). The number of samples represented in each group is indicated.