A. Targeted gene expression profile of 96 genes by multiplex qPCR involved in cell cycle, transcription factors, CD8 effector functions, and TCR signaling pathways for the CD8+ T cells in the CSF of AHI stage ½ (n=9), AHI stage 3 (n=14), and CHI (n=9) participants compared to uninfected participants (n=6). Scale represents fold change in gene expression of 100 total CD8+ T cells in the analyzed group compared to uninfected participants. Forty-two genes were significantly different (p<0.05) are displayed in heatmap based on one-way ANOVA with Geisser-Greenhouse correction and Tukey-Kramer’s post-hoc test. B. Graphs represent relative mRNA expression for individual subjects for select genes from each pathway. AHI: acute HIV-infection, CHI: chronic HIV-infection.