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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: New Phytol. 2017 Jan 20;214(3):1145–1157. doi: 10.1111/nph.14422

Fig. 6.

Fig. 6

Characterization of phytochromes incorporating phycocyanobilin (PCB) produced by KflaHY2. (a) Absorption spectra are shown for Cph1 in the Pr (blue) and Pfr (orange) photostates. KflaHY2 was used to drive bilin biosynthesis, and protein was expressed as a poly-His construct. (b) Normalized difference spectra are shown for Cph1 expressed using cyanobacterial PcyA (blue) or KflaHY2 (red) for bilin biosynthesis. (c) Absorption spectra are shown for MvirPHY1 in the Pr (blue) and Pfr (orange) photostates. Cyanobacterial PcyA was used to drive PCB biosynthesis, and protein was expressed as an intein-chitin-binding-domain (intein-CBD) fusion protein. (d) Absorption spectra are shown for MvirPHY1 in the Pr (blue) and Pfr (orange) photostates. KflaHY2 was used to drive bilin biosynthesis, and protein was expressed as a poly-His construct. (e) Normalized difference spectra are shown for MvirPHY1 expressed using cyanobacterial PcyA (blue) or KflaHY2 (red) for bilin biosynthesis.