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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: New Phytol. 2017 Jan 20;214(3):1145–1157. doi: 10.1111/nph.14422

Table 1.

In vitro spectral properties of biliprotein photosensors in this study1

Photoreceptor Affinity Tag FDBR SAR 15Z λmax 15E λmax
NpR5113g22 intein-CBD PcyA 1.2 650 528
NpR5113g2 intein-CBD AtHY2 0.4 664 542
NpR5113g2 poly-His KflaHY2 0.5 650 530
NpR5113g33 poly-His PcyA 0.3 422 496
NpR5113g23 poly-His PcyA 0.4 650, 422 528, 496
NpR5113g23 poly-His KflaHY2 0.2 650, 424 528, 496
Cph1 poly-His PcyA 0.2 660 704
Cph1 poly-His KflaHY2 0.1 666 714
MvirPHY1 intein-CBD PcyA 0.9 646 718
MvirPHY1 poly-His KflaHY2 0.1 646 716
1

For NpR5113g23, values for domain 2 are reported first and values for domain 3 are derived from the photochemical difference spectrum after subtraction of signals from domain 2 (Fig. 5). Specific absorbance ratio (SAR) was calculated as the ratio of the peak absorbance of the longest-wavelength chromophore band for the 15Z photostate to the peak absorbance of the aromatic amino acid band at 280 nm. NpR5113g2 was expressed in the presence of KflaHY2 and KflaHY1, whereas other KflaHY2 expressions used KflaHY2 and cyanobacterial heme oxygenase. 2Values from Rockwell et al. (2012c). 3Values from Rockwell et al. (2012a).