Table 2.
Frequencies of six CTLA-4 gene variants in 173 metastatic melanoma patients and in healthy control subjects.
|
n (%) |
n (frequency) |
||||||
|---|---|---|---|---|---|---|---|
| Genotypes | Melanoma patients (n = 173) | Controls (n = 100) | P¶ | Alleles | Melanoma patients (2n = 346) | Controls (2n = 200) | P* |
| −1661A>G | −1661A>G | ||||||
| A/A | 102 (59.0) | 58 (58.0) | 0.868 | A | 266 (0.77) | 154 (0.77) | 1.000 |
| A/G | 62 (35.8) | 38 (38.0) | G | 80 (0.23) | 46 (0.23) | ||
| G/G | 9 (5.2) | 4 (4.0) | |||||
| HWE | P = 0.915 | P = 0.466 | |||||
| −1577G>A | −1577G>A | ||||||
| G/G | 49 (28.3) | 26 (26.0) | 0.807 | G | 186 (0.54) | 102 (0.51) | 0.535 |
| G/A | 88 (50.9) | 50 (50.0) | A | 160 (0.46) | 98 (0.49) | ||
| A/A | 36 (20.8) | 24 (24.0) | |||||
| HWE | P = 0.761 | P = 0.997 | |||||
| −658C>T | −658C>T | ||||||
| C/C | 139 (80.3) | 76 (76.0) | 0.669 | C | 310 (0.89) | 175 (0.87) | 0.482 |
| C/T | 32 (18.5) | 23 (23.0) | T | 36 (0.11) | 25 (0.13) | ||
| T/T | 2 (1.2) | 1 (1.0) | |||||
| HWE | P = 0.917 | P = 0.607 | |||||
| −319C>T | −319C>T | ||||||
| C/C | 139 (80.3) | 81 (81.0) | 0.517 | C | 311 (0.90) | 179 (0.89) | 0.884 |
| C/T | 33 (19.1) | 17 (17.0) | T | 35 (0.10) | 21 (0.11) | ||
| T/T | 1 (0.6) | 2 (2.0) | |||||
| HWE | P = 0.519 | P = 0.339 | |||||
| +49A>G | +49A>G | ||||||
| A/A | 96 (55.5) | 55 (55.0) | 0.780 | A | 255 (0.74) | 149 (0.75) | 0.919 |
| A/G | 63 (36.4) | 39 (39.0) | G | 91 (0.26) | 51 (0.25) | ||
| G/G | 14 (8.1) | 6 (6.0) | |||||
| HWE | P = 0.425 | P = 0.791 | |||||
| CT60G>A | CT60G>A | ||||||
| G/G | 42 (24.3) | 24 (24.0) | 0.877 | G | 173 (0.50) | 97 (0.48) | 0.790 |
| G/A | 89 (51.4) | 49 (49.0) | A | 173 (0.50) | 103 (0.52) | ||
| A/A | 42 (24.3) | 27 (27.0) | |||||
| HWE | P = 0.748 | P = 0.848 | |||||
Genotyping of −1661A>G, −1577A>G, −658C>T, and CT60G>A was performed by the pyrosequencing (PSQ) method and genotyping of −319C>T and +49A>G was performed by T-ARMS PCR and confirmed by PSQ as described in the Section “Materials and Methods”. Comparison of CTLA-4 genotypic and allelic frequencies between melanoma patients and control subjects was estimated using the Pearson’s χ2 test (¶P-value) and the Fisher’s test (*P-value), respectively. Statistical significance: P < 0.05. HWE, Hardy–Weinberg equilibrium tested by χ2 test (P < 0.05 indicates a lack of HWE).