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. 2017 Apr 12;8:648. doi: 10.3389/fmicb.2017.00648

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Copyright © 2017 Ruscitto, Honma, Veeramachineni, Nishikawa, Stafford and Sharma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reporter assay for detection of AmpG permease activity. The β-lactamase assay was performed on Escherichia coli strains BW25113 (parental strain), TP73 (ΔampD)/pNU305, AR74 (ΔampG/ΔampD)/pNU305/ pACY-AR1 (empty vector), and AR74/pNU305/pAC-Tanf_08635. Activity was determined by chromogenic nitrocefin substrate induced at 0, 30, 60 min and absorbance was measured at OD₄₈₆. Data are representative of three independent experiments with similar results. Each value represents the mean (±SD) of three values measured in one representative assay.