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. 2017 Apr 12;8:648. doi: 10.3389/fmicb.2017.00648

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Copyright © 2017 Ruscitto, Honma, Veeramachineni, Nishikawa, Stafford and Sharma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of AmpG and MurNAc utilization operon. RT-PCR analysis with (A) primer sets spanning adjacent genes (fragments a-j). Tanf_08345, xanthane lyase; Tanf_08350 (gtf), glycosyltransferase; Tanf_08355 (gtf), glycosyltransferase; Tanf_08360 (lytB), amidase enhancer precursor; Tanf_08365 (ampG), muropeptide permease; Tanf_08370 (ybbC), conserved hypothetical protein. (B) PCR products were separated on a 1% agarose gel. RNA samples with no reverse transcription reaction as template controls were run in lanes 1, genomic DNA as template in lanes 2, and cDNA as template for each primer set in lanes 3. MW; DNA ladder. (C) DNA sequence showing transcriptional start site determine by 5′RACE.