Table 1.
Overview of some plasmid-based AHL reporter strains (Steindler and Venturi, 2007; Rai et al., 2015; O'Connor et al., 2016).
| Plasmid | Bacterial strain | Analyte | Promoter | Reporter |
|---|---|---|---|---|
| pSB401 | E. coli | 3-oxo-C4- to 3-oxo-C14-HSL C4- to C12-HSL | luxI | luxCDABE |
| pSB1075 | E. coli | 3-oxo-C12- to 3-oxo-C16-HSL C12- to C16-HSL | lasI | luxCDABE |
| pECP61.5 | P. aeruginosa | C4-HSL | rhlA | lacZ |
| pKDT17 | E. coli | 3-oxo-C10- to 3-oxo-C12-HSL C10- to C12-HSL | lasB | lacZ |
| pCF218, pMV26 | A.tumefaciens | 3-oxo-C6- to 3-oxo-C12-HSL C4- to C12-HSL | traI | luxCDABE |
| pCF218, pCF372 | A.tumefaciens | 3-oxo-C4- to 3-oxo-C12-HSL C5- to C10-HSL | traI | lacZ |
| pSB406 | E. coli | 3-oxo-C4- to 3-oxo-C14-HSL C4- to C12-HSL | rhlI | luxCDABE |
| C. violaceum | 3-oxo-C6- to 3-oxo-C8-HSL C4- to C8-HSL | cviI | Violacein | |
| pAL105 | E. coli | 3-oxo-C12-HSL | lasI | luxCDABE |
| pAL101 | E. coli | C4-HSL | rhlI | luxCDABE |
| pSB536 | E. coli | C4-HSL | ahyI | luxCDABE |
| pSB403 | Broad host range | 3-oxo-C4- to 3-oxo-C14-HSL C4- to C12-HSL | luxI | luxCDABE |
| pHV2001 | E. coli | 3-oxo-C6- to 3-oxo-C8-HSL C6- to C8-HSL | luxI | luxCDABE |
| pZLR4 | A.tumefaciens | All 3-oxo-HSLs C6- to C14-HSL 3-OH-C6- to 3-OH-C10-HSL | traI | lacZ |
| pJZ384, pJZ410, pJZ372 | A.tumefaciens | 3-oxo-C4- to 3-oxo-C18-HSL C4- to C18-HSL | traI | lacZ |
| pSF105, pSF107 | P. fluorescens | 3-OH-C6-HSL C6-HSL 3-OH-C8-HSL | phzI | lacZ |
| pUCP18 | P. aeruginosa | 3-oxo-C12-HSL | rsaL | luxCDABE |
| pMS402 | P. aeruginosa | 3-oxo-C12-HSL | rsaL | luxCDABE |
| pUCGMAT1-4 | E. coli | 3-oxo-C6-HSL | ahlI | mcherry |
| pREC-FF | E. coli | 3-oxo-C6-HSL | luxI | cfp |
| M71LZ | P. aeruginosa lasI − | 3-oxo-C10 to 3-oxo-C12-HSL | lasI | lacZ |
| pAS-C8 | Broad host range | C8- to C10-HSL | cepI | gfp |
| pKR-C12 | Broad host range | 3-oxo-C10- to 3-oxo-C12-HSL | lasI | gfp |
| pJBA-132 | Broad host range | 3-oxo-C6-HSL C6- to C10-HSL | luxI | gfp |