Figure 6.

Growth and enterotoxin production of 7 selected B. cereus strains comparing CaCo-2 treated cRPMI medium with untreated RPMI 1640 medium. Both media types were supplemented with 2% casein hydrolysate and 1% glucose. cRPMI medium had been pre-incubated for 22 h with differentiated CaCo-2 cells. Bacteria were grown in 80 cm² cell culture flasks at 37°C under 7% CO₂ atmosphere. OD₆₀₀ was recorded for 10 h and every 2 h samples of the supernatant were taken. Enterotoxin production was determined via sandwich EIA specific for NheB.