Figure 2.

Substitution analysis for Apidaecin (GNNRPVYIPQPRPPHPRL). The original sequence and amino acid positions are given in the first two columns. The other rows (A–Y) identifies the amino acid replacements at each position. Each box in the matrix corresponds to a single peptide containing an additional glycine at the C-terminus. The values within each box represent a RelIC₇₅ value, determined by treatment of the Pseudomonas aeruginosa reporter strain H1001 with any given peptide for 4 h. Boxes are color-coded by a dynamic range between blue and red: blue stands for improved activity compared to the parent peptide, green for similar activity, and red indicates no activity. Empty boxes represent the original sequence.