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. 2017 Apr 12;8:220. doi: 10.3389/fphys.2017.00220

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Copyright © 2017 Yang, Ahmari, Schmidt, Redler, Arocha, Pacholec, Magee, Malphurs, Owen, Krane, Li, Wang, Vickroy, Raizada, Martyniuk and Zubcevic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Heatmap of differentially expressed probes (unadjusted, P < 0.05) between C57 and b1/2-ARs KO chimera. (A) Validation of microarray data by real time PCR. Three genes were selected for validation of microarray analyses. Bar graph shows relative expression level of gene normalized to GAPDH expression. (B) There was a clear distinction between all individuals from each group, with two expression clades evident. (C) The three transcripts with the most significant differences between two groups in the microarray analyses are identified as Klrd1, Ms4a4b and Csnk2a2, presented in the bar graph. Two tailed unpaired t-test analysis indicates ^****P < 0.0001. Adjusted P-values are 0.0042, 0.0085, and 0.0216, respectively. ^*indicated unpaired t-test P < 0.05.