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. 2017 Apr 12;8:220. doi: 10.3389/fphys.2017.00220

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Copyright © 2017 Yang, Ahmari, Schmidt, Redler, Arocha, Pacholec, Magee, Malphurs, Owen, Krane, Li, Wang, Vickroy, Raizada, Martyniuk and Zubcevic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Decreased infiltration of particular IC subsets in the colons of the b1/2-ARs KO chimera. Significantly lower levels of CD4⁺ cells (A), CD11b⁺ macrophages (B) and CD94⁺ NK cells (C) were observed in the colons of b1/2-ARs KO chimeras compared with C57 control chimeras. Quantified data were presented in the (D). Blue indicates non-specific DAPI staining, while red shows CD4⁻, CD11b⁻, and CD94⁻ positive cells, respectively. Images were obtained at 400X magnification. All bars indicate 20 μm. Unpaired two tailed t-test showed ^***P < 0.001 and ^****P < 0.0001.