Table 1.
Primer sequences for microbiota 16S V4–5 amplification.
| Name | Forward primer | Reverse primer |
|---|---|---|
| C1 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGA CGCTCTTCCGATCTTAGCTGCCAGCMGCCGCGGTAAT |
CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAA CCGCTCTTCCGATCTGTAGCACCGTCAATTYYTTTRAGTTT |
| C2 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGAC GCTCTTCCGATCTAGCTACCCAGCMGCCGCGGTAAT |
CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCG CTCTTCCGATCTGTAGCACCGTCAATTYYTTTRAGTTT |
| C3 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGAC GCTCTTCCGATCTAGCTACCCAGCMGCCGCGGTAAT |
CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAA CCGCTCTTCCGATCTCACTGTCCGTCAATTYYTTTRAGTTT |
| C4 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTAC ACGACGCTCTTCCGATCTAGCTACCCAGCMGCCGCGGTAAT |
CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTG AACCGCTCTTCCGATCTATTGGCCCGTCAATTYYTTTRAGTTT |
| C6 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTAC ACGACGCTCTTCCGATCTAGCTACCCAGCMGCCGCGGTAAT |
CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACC GCTCTTCCGATCTGATCTGCCGTCAATTYYTTTRAGTTT |
| C7 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACG ACGCTCTTCCGATCTAGCTACCCAGCMGCCGCGGTAAT |
CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACC GCTCTTCCGATCTAAGCTACCGTCAATTYYTTTRAGTTT |
| KO1 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTAC ACGACGCTCTTCCGATCTTAGCTGCCAGCMGCCGCGGTAAT |
CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGA ACCGCTCTTCCGATCTTACCATCCGTCAATTYYTTTRAGTTT |
| KO2 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACA CGACGCTCTTCCGATCTAGCTACCCAGCMGCCGCGGTAAT |
CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACC GCTCTTCCGATCTCGTGATCCGTCAATTYYTTTRAGTTT |
| KO3 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACG ACGCTCTTCCGATCTAGCTACCCAGCMGCCGCGGTAAT |
CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTG CTGAACCGCTCTTCCGATCTACATCGCCGTCAATTYYTTTRAGTTT |
| KO4 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACAC GACGCTCTTCCGATCTAGCTACCCAGCMGCCGCGGTAAT |
CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAA CCGCTCTTCCGATCTGCCTAACCGTCAATTYYTTTRAGTTT |
| KO5 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTA CACGACGCTCTTCCGATCTAGCTACCCAGCMGCCGCGGTAAT |
CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCG CTCTTCCGATCTTCAAGTCCGTCAATTYYTTTRAGTTT |
| KO6 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGAC GCTCTTCCGATCTAGCTACCCAGCMGCCGCGGTAAT |
CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTG AACCGCTCTTCCGATCTCTGATACCGTCAATTYYTTTRAGTTT |