Figure 3.

Model training and validation using cell proliferation data. The model is used to estimate the relative number of cells. (A) Simulated cell proliferation with complete media (35 mM glucose and 6 mM glutamine) for 5 days with no knockdown (black) and under GOT1 knockdown (red). Results are shown for the four initial conditions that match the training data. (B) Simulated cell proliferation when cells are grown in complete media for 5 days (black) or in complete media for 24 h, followed by glucose and glutamine deprivation for 4 days (blue). Results are shown for the final two initial conditions that match the validation data. In both (A,B), triangles and squares represent the experimental data with error bars as available. The solid lines indicate the mean of predicted results for the given sets of initial conditions, and the shading shows the standard deviation.