TABLE 2.
Antimicrobial activities of human α-defensins against six strains
| Test organism | vLD50 (μg/ml [mean ± SEM])a
|
vLD90 (μg/ml [mean ± SEM])a
|
vLD99 (μg/ml [mean ± SEM])a
|
vLD99.9 (μg/ml [mean ± SEM])a
|
||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HNP-1 | HNP-2 | HNP-3 | HNP-4 | HD5 | HD6 | HNP-1 | HNP-2 | HNP-3 | HNP-4 | HD5 | HD6 | HNP-1 | HNP-2 | HNP-3 | HNP-4 | HD5 | HD6 | HNP-1 | HNP-2 | HNP-3 | HNP-4 | HD5 | HD6 | |
| E. coli ATCC 8739 | 3.6 ± 0.3 | 3.5 ± 0.7 | 6.2 ± 0.9 | 3.3 ± 0.4 | 2.5 ± 0.3 | >256 | 14 ± 4.3 | 20 ± 7.3 | 61 ± 25 | 4.9 ± 1.0 | 4.5 ± 0.9 | >256 | 106 ± 4.8b | 49 ± 11 | 163 ± 41 | 7.5 ± 0.3 | 5.9 ± 1.2 | >256 | ||||||
| E. coli ATCC 25922 | 3.7 ± 0.4 | 3.5 ± 0.6 | 5.9 ± 2.1 | 3.0 ± 0.7 | 2.1 ± 0.9 | 103 ± 14 | 9.4 ± 0.9 | 8.4 ± 1.3 | 23 ± 4.2 | 4.8 ± 1.1 | 3.6 ± 0.8 | >256 | >256 | 19 ± 4.4 | 93 ± 43 | 7.0 ± 0.5 | 5.3 ± 1.3 | >256 | ||||||
| E. aerogenes ATCC 13048 | 10 ± 0.5b | 16 ± 4.0 | 41 ± 9.2 | 6.6 ± 0.2 | 5.5 ± 0.5 | 156 ± 11b | 70 ± 35b | 26 ± 1.7b | 82 ± 19b | 12 ± 0.8b | 7.6 ± 0.04b | >256 | >256 | >256 | >256 | 12 ± 0.8b | >256 | >256 | ||||||
| S. aureus ATCC 25923 | 4.2 ± 1.0 | 4.2 ± 0.9 | 13 ± 2.1 | 7.3 ± 1.4 | 6.3 ± 0.5 | >256 | 21 ± 0.8 | 18 ± 1.2 | 52 ± 5.9 | >256 | 24 ± 1.1 | >256 | 48 ± 3.1 | 37 ± 6.0 | 117 ± 9.9 | >256 | 58 ± 0.7 | >256 | ||||||
| S. aureus ATCC 29213 | 2.1 ± 0.3 | 2.4 ± 0.3 | 2.2 ± 0.4 | 7.2 ± 0.2 | 3.2 ± 0.3 | >256 | 7.0 ± 0.4 | 9.4 ± 1.1 | 16 ± 2.1 | 14 ± 0.2 | 7.0 ± 0.4 | >256 | 17 ± 0.7 | 22 ± 1.0 | 46 ± 8.8 | 29 ± 0.3 | 13 ± 1.1 | >256 | ||||||
| B. cereus ATCC 10876 | 0.22 ± 0.03 | 0.22 ± 0.04 | 0.37 ± 0.08 | 0.87 ± 0.08 | <0.31 | 25 ± 13 | 0.82 ± 0.09 | 0.79 ± 0.09 | 0.74 ± 0.2 | 2.5 ± 0.5 | 0.44 ± 0.1 | 100 ± 49 | 1.9 ± 0.04 | 1.8 ± 0.1 | 1.6 ± 0.3 | 5.1 ± 1.1 | 2.3 ± 0.8 | 240 ± 7.9 | 3.9 ± 0.03 | 3.9 ± 0.02 | 4.1 ± 0.13 | 12 ± 1.8 | 7.5 ± 0.2 | >256 |
a
The values are for three replicates of each experiment unless indicated otherwise.
b
The value for one of the three replicates was >256 μg/ml; in these cases, the standard error of the mean is reported for n = 2. When one of the three replicates was determinable and the other two were >256 μg/ml, a value of >256 μg/ml was entered.