Maximum parsimony tree of 186 global Burkholderia pseudomallei isolates. Gray shading represents isolates from CF patients, 18 of which were sequenced in this study and 2 by others (Bp1651 [8] and 4900CFPatient1 [9]). Text color denotes geographic origin (red, Australia; blue, Asia; gold, South America). This analysis demonstrates that longitudinally collected isolates from within patients are genetically related; however, there is no distinct “CF lineage.” This analysis also confirms that CF11 was infected while traveling in Thailand; all other patients in our study acquired their infections in Australia. Branches with <85% bootstrap support are labeled. Consistency index, 0.25. Root taxon, MSHR0668.