TABLE 2.
TMT-MS analysis results for wild-type versus Rv2125 mutant M. tuberculosisa
| Protein | Protein description | No. of peptides | WT avg | Mutant avg | Fold change, mutant/WT |
|---|---|---|---|---|---|
| Rv2125.1 | 5 | 32.04 | 1.29 | 0.04 | |
| Proteins with >2-fold decrease | |||||
| Rv0011c.1 | CrgA | 1 | 28.75 | 4.58 | 0.16 |
| Rv1131.1 | Methylcitrate synthase PrpC | 2 | 28.18 | 5.16 | 0.18 |
| Rv1130.1 | Methylcitrate dehydratase PrpD | 13 | 27.25 | 6.08 | 0.22 |
| Rv1606.1 | Phosphoribosyl-AMP cyclohydrolase | 3 | 26.31 | 7.02 | 0.27 |
| Rv0863.1 | Hypothetical | 1 | 25.23 | 8.10 | 0.32 |
| Rv1624c.1 | Hypothetical | 1 | 24.43 | 8.90 | 0.36 |
| Rv1660.1 | Chalcone synthase Pks10 | 5 | 24.38 | 8.96 | 0.37 |
| Rv0367c.1 | Hypothetical | 1 | 24.19 | 9.14 | 0.38 |
| Rv2930.1 | Fatty acid coenzyme A ligase FadD | 9 | 22.89 | 10.44 | 0.46 |
| Rv3364c.1 | Hypothetical | 2 | 22.80 | 10.54 | 0.46 |
| Proteins with >2-fold increase | |||||
| Rv3875.1 | Early secretory antigenic target EsxA | 2 | 11.11 | 22.23 | 2.00 |
| Rv2628.1 | Hypothetical | 1 | 11.05 | 22.29 | 2.02 |
| Rv2055c.1 | 30S ribosomal protein S18 RpsR2 | 6 | 10.96 | 22.37 | 2.04 |
| Rv0106.1 | Hypothetical | 1 | 10.79 | 22.54 | 2.09 |
| Rv0572c.1 | Hypothetical | 4 | 10.55 | 22.79 | 2.16 |
| Rv2558.1 | Monooxygenase | 7 | 10.37 | 22.96 | 2.21 |
| Rv2056c.1 | Ribosomal protein | 2 | 8.08 | 25.26 | 3.13 |
| Proteins of the Pup-20S system | |||||
| Rv2097c.1 | Pup ligase PafA | 36 | 16.16 | 17.17 | 1.06 |
| Rv2109c.1 | 20S α subunit PrcA | 18 | 16.74 | 16.59 | 0.99 |
| Rv2110c.1 | 20S β subunit PrcB | 20 | 17.62 | 15.71 | 0.89 |
| Rv2111c.1 | Pup | 4 | 16.73 | 16.61 | 0.99 |
| Rv2112c.1 | Depupylase Dop | 26 | 16.65 | 16.69 | 1.00 |
| Rv2115c.1 | Mpa | 39 | 16.71 | 16.63 | 1.00 |
| Rv3780.1 | PafE | 24 | 16.12 | 17.22 | 1.07 |
a
Listed are proteins whose relative abundance is more than 2-fold higher or lower in the Rv2125 mutant (Rv2125::ΦMycoMarT7) strain.