FIG 4.
Expression of PilA, HmpD, and HPS in the ΔogtA mutant strain. (A) Western blot analysis of HmpD and PilA in hormogonia of the wild-type, ΔhmpD mutant, and ΔogtA mutant strains. (B) Quantification of pilA expression by using RT-qPCR and a pilA promoter region-gfp reporter construct (PpilA-sfgfp-aav in pBK109) in the wild-type or ΔogtA mutant strain 0 and 24 h post-hormogonium induction. Values are expressed as a percentage of the expression level in wild-type hormogonia 24 h postinduction (n = 3; error bars = 1 SD). (C) Quantification of the cell surface and soluble fractions of HPS in the wild-type, ΔhpsE–D mutant, ΔpilA mutant, and ΔogtA mutant strains. Values are expressed as a percentage of the fluorescence (surface fraction) or chemiluminescent (soluble fraction) intensity for wild-type hormogonia 24 h postinduction (n = 3; error bars = 1 SD).