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. 2017 Apr 12;7:46404. doi: 10.1038/srep46404

Figure 3. Effect of restricted feeding on adrenal StAR expression and corticosterone levels.

Figure 3

(a and b) Adrenal StAR protein levels in freely fed (FF) and restricted fed (RF) mice as determined by immunoblotting. Mice subjected to restricted feeding were sacrificed on day 3 (2–3 d) or day 7 (6–7 d). Restricted fed (starved) mice were sacrificed on day 7 without food on the last day to examine the possible effect of hunger on StAR expression (a). Optical band intensities for FF and RF mice (6–7 d) are expressed as mean ± SE of arbitrary units (A.U.), with values from FF mice at ZT00 defined as 1 (b; n = 3; *p < 0.05 vs. FF at the same time-of-day). (c and d) Effects of restricted feeding on daily rhythm of adrenal corticosterone content (c; n = 7–10; *p < 0.05 and **p < 0.01 vs. FF at the same time-of-day) and circulating corticosterone levels in plasma (d; n = 4–5; **p < 0.01 vs. FF at the same time-of-day) as measured by radioimmunoassay.