Table 1. Saccharomyces cerevisiae strains used in this study.

Strain	Relevant genotype/description	Reference
CEN.PK 113-7D	MATa MAL2-8c SUC2	55
IMX581	MATa ura3-52 MAL2-8c SUC2 can1Δ::cas9-natNT2	33
IMX696	MATa ura3-52 MAL2-8c SUC2 CAN1::cas9-natNT2 gre3:: [pTDH3_RPE1- pPGK1_TKL1- pTEF1_TAL1- pPGI1_NQM - pTPI1_RKI1- pPYK1_TKL2- (pTPI1_xylA_tCYC1)*36 pTEF1_XKS1] pUDE335	This study
IMS0488	Single-cell line isolated after adaptation of IMX696 to anaerobic growth on xylose (reactor 1)	This study
IMS0489	Single-cell line isolated after adaptation of IMX696 to anaerobic growth on xylose (reactor 2)	This study
IMX906	MATa ura3-52 MAL2-8c SUC2 CAN1::cas9-natNT2 gre3:: [pTDH3_RPE1- pPGK1_TKL1- pTEF1_TAL1- pPGI1_ NQM1-pTPI1_RKI1- pPYK1_TKL2- (pTPI1_xylA_tCYC1)*36 pTEF1_XKS1] pmr1Δ::amdSYM pUDE335	This study
IMX979	IMX906 with PMR1 reintegrated at PMR1 locus	This study
IMK692	MATa MAL2-8c SUC2 pmr1Δ::amdSYM	This study

Native gene terminator sequences were used for expression of RPE1, TKL1, TAL1, NQM1, RKI1, TKL2 and XKS1.