Table 3. Impact of the deletion of PMR1 on intracellular metal ion concentrations.
| S. cerevisiae strain (relevant genotype) | Carbon source | μmol (g dry biomass)−1 | ||||
|---|---|---|---|---|---|---|
| Mg2+ | Ca2+ | Fe2+ | Mn2+ | Total | ||
| IMX696 (xylA, PPP↑, XKS1↑) | glucose | 60 ± 1 | 8.8 ± 1.6 | 0.56 ± 0.02 | 0.069 ± 0.001 | 69 ± 2 |
| IMX906 (xylA, PPP↑, XKS1↑, pmr1Δ) | glucose | 64 ± 1 | 12 ± 2 | 0.51 ± 0.02 | 1.2 ± 0.1 | 77 ± 2 |
| IMX906 (xylA, PPP↑, XKS1↑, pmr1Δ) | xylose | 50 ± 1 | 4.0 ± 0.7 | 0.36 ± 0.01 | 1.4 ± 0.1 | 56 ± 1 |
| CEN.PK113-7D (reference strain) | glucose | 61 ± 1 | 6.8 ± 1.3 | 0.44 ± 0.01 | 0.046 ± 0.001 | 68 ± 2 |
| IMK692 (pmr1Δ) | glucose | 72 ± 1 | 6.7 ± 1.2 | 0.46 ± 0.02 | 0.86 ± 0.06 | 80 ± 2 |
S. cerevisiae strains were grown in anaerobic bioreactors on xylose or glucose (20 g l−1). Data represent average and mean deviation calculated from analyses on independent duplicate cultures.