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| Metal | Kinetic parameters | ||
|---|---|---|---|
| kcat (s−1) | KM (mM) | kcat/KM (s−1 · M−1) | |
| Mg2+ | 2.8 ± 0.2 | 5.5 ± 0.4 | 500 |
| Mn2+ | 7.8 ± 0.1 | 3.9 ± 0.2 | 2000 |
| Ca2+ | 0.6 ± 0.06 | 420 ± 90 | 1.3 |
kcat and KM values represent average and mean deviation of independent duplicate experiments, calculated for each metal.
