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. 2017 Apr 12;7:46220. doi: 10.1038/srep46220

Figure 1. Propagation of mesenchymal stem cells (MSCs) derived from human term placental tissue.

Figure 1

(a) Gross anatomical (left) and schematic (right) images of term placenta. Tissues from chorionic plate (CP), chorionic villi (CV), and decidua basalis (DB) were separated manually and collected for ex vivo expansion of MSCs. (b) Phase-contrast microscopic images showing outgrowth of fibroblast-like cells from explants of CP (CP-MSCs), CV (CV-MSCs), and DB (DB-MSCs) at about 10 days after initiation of cultures. Scale bars: 200 μm. (c) Growth kinetics of twice-passaged CP-MSCs, CV-MSCs, and DB-MSCs. (d) Representative electrophoretogram of microsatellite genotyping using representative short tandem repeat markers D8S1179, TH01, vWs, and amelogenin. CP-MSC and CV-MSC matched the baby (cord blood), while DB-MSC matched the mother. Amelogenin confirmed the presence of the X chromosome-specific allele alone for DB-MSC (mother), and both X and Y chromosome-specific alleles for CP-MSC and CV-MSC (baby).