Figure 2. Characteristics of mesenchymal stem cells (MSCs) primarily expanded from human term placental tissue.

Twice-passaged MSCs from chorionic plate (CP-MSCs), chorionic villi (CV-MSCs), and decidua basalis (DB-MSCs) were used for experiments. (a) Flow cytometry analysis showing expression profiles of cell surface markers on cells (grey areas indicated isotype negative controls). (b) Immunocytochemical images showing CP-MSCs, CV-MSCs, and DB-MSCs positively stained for the mesenchymal marker vimentin (green) but negatively stained for the pan-trophoblast marker keratin-7 (red). Nuclei were counterstained with DAPI (blue). BeWo trophoblast cells were used as control. Scale bars: 50 μm.